通过尺寸排阻色谱法深入了解透明质酸和一些β-(1→3)-葡聚糖的分子量分布及高阶结构

Insight into the distribution of molecular weights and higher-order structure of hyaluronans and some beta-(1 --> 3)-glucans by size exclusion chromatography.

作者信息

Soltés L, Mislovicová D, Sébille B

机构信息

Institute of Experimental Pharmacology, Slovak Academy of Sciences, Bratislava, Slovak Republic.

出版信息

Biomed Chromatogr. 1996 Mar-Apr;10(2):53-9. doi: 10.1002/(SICI)1099-0801(199603)10:2<53::AID-BMC552>3.0.CO;2-5.

DOI:10.1002/(SICI)1099-0801(199603)10:2<53::AID-BMC552>3.0.CO;2-5

PMID:8924726

Abstract

The effects of high energy ultrasound and slightly raised temperature combined with the denaturing action of dimethylsulphoxide on the molecular weight and higher-order structure of hyaluronans and some beta-(1 --> 3)-glucans were studied by means of size exclusion chromatography (SEC) technique. Some experimental conditions connected with the (bio-)polymer sample preparation prior to its SEC analysis are overviewed in the light of informational relevance of studies where the action of a physical and/or chemical agent changes the hydrodynamic size of the m omolecule.

摘要

采用尺寸排阻色谱（SEC）技术，研究了高能超声、微热以及二甲基亚砜的变性作用对透明质酸和一些β-(1→3)-葡聚糖的分子量及高级结构的影响。鉴于在物理和/或化学试剂作用改变分子流体力学尺寸的研究中信息的相关性，对在SEC分析之前与（生物）聚合物样品制备相关的一些实验条件进行了概述。