Strobel E
Institut für Medizinische Mikrobiologie, Immunologie und Krankenhaushygiene, Städtisches Krankenhaus Müchen-Schwabing, Deutschland.
Infusionsther Transfusionsmed. 1996 Jun;23(3):138-42.
Weak expression of ABO antigens in newborns has been known for many years. In the near future reduced availability of polyclonal sera for ABO testing is to be expected. Therefore, it was the aim of our study to compare the suitability of monoclonal reagents for ABO testing of newborns to that of polyclonal sera.
One monoclonal and 1 polyclonal reagent of each of the specificities anti-A, and anti-B and anti-AB from 7 manufacturers were tested by titration with blood from 5 newborns of blood group A, 5 of blood group B, and 3 of blood group AB.
All monoclonal anti-A reagents showed better results than the polyclonal sera (for newborns of blood group A on the average of the 7 pairs of reagents 1.3 geometrical titration steps). Most of the monoclonal anti-B reagents showed higher titers than the polyclonal sera (on the average for newborns of blood group B 0.7 titration steps). Also most monoclonal anti-AB reagents were stronger than the polyclonal sera in testing newborns of blood group B the monoclonal anti-AB-reagents were slightly weaker than the polyclonal sera (on the average 0.4 titration steps).
Use of monoclonal reagents for ABO testing of newborns shows some advantages in comparison to the use of polyclonal sera.
新生儿ABO抗原表达较弱已为人所知多年。在不久的将来，预计用于ABO检测的多克隆血清供应将减少。因此，我们研究的目的是比较单克隆试剂与多克隆血清用于新生儿ABO检测的适用性。
用来自5名A型血新生儿、5名B型血新生儿和3名AB型血新生儿的血液进行滴定，对7家制造商生产的抗A、抗B和抗AB特异性的1种单克隆试剂和1种多克隆试剂进行检测。
所有单克隆抗A试剂的检测结果均优于多克隆血清（对于A型血新生儿，7对试剂的平均值为1.3个几何滴定步长）。大多数单克隆抗B试剂的效价比多克隆血清高（对于B型血新生儿，平均值为0.7个滴定步长）。在检测B型血新生儿时，大多数单克隆抗AB试剂也比多克隆血清更强，单克隆抗AB试剂略弱于多克隆血清（平均值为0.4个滴定步长）。
与使用多克隆血清相比，使用单克隆试剂进行新生儿ABO检测具有一些优势。
