人血浆中L-丁硫氨酸-（R，S）-亚砜亚胺非对映异构体的定量分析：一种经过验证的毛细管电泳测定法

Quantitation of the diastereoisomers of L-buthionine-(R,S)-sulfoximine in human plasma: a validated assay by capillary electrophoresis.

作者信息

Sandor V, Flarakos T, Batist G, Wainer I W, Lloyd D K

机构信息

Department of Oncology, McGill University, Montreal, PQ, Canada.

出版信息

J Chromatogr B Biomed Appl. 1995 Nov 3;673(1):123-31. doi: 10.1016/0378-4347(95)00242-b.

DOI:10.1016/0378-4347(95)00242-b

PMID:8925065

Abstract

An assay for the diastereoisomers of the biochemical modifier L-buthionine-(R,S)-sulfoximine (BSO) in human plasma has been developed using capillary electrophoresis (CE). Separation of the diastereoisomers is achieved by the micellar electrokinetic chromatography (MEKC) mode of CE. Plasma is injected directly onto the separation capillary without any extraction step, and BSO is detected directly by ultraviolet absorbance measurements at 190 nm without prior derivatization. The whole assay, including capillary conditioning, takes approximately 30 min. Intra- and inter-day R.S.D. values are approximately 7% at sample concentrations around 25 micrograms ml-1, and approximately 3% at sample concentrations around 500 micrograms ml-1. The limit of detection in plasma is 3.9 micrograms ml-1 (S/N = 2). The assay has been used to quantitate the diastereoisomers of BSO in patient samples in a pharmacokinetic study.

摘要

已开发出一种利用毛细管电泳（CE）测定人血浆中生化修饰剂L-丁硫氨酸-（R，S）-亚砜亚胺（BSO）非对映异构体的方法。通过CE的胶束电动色谱（MEKC）模式实现非对映异构体的分离。血浆无需任何萃取步骤即可直接进样到分离毛细管中，并且无需预先衍生化，直接通过190nm处的紫外吸光度测量来检测BSO。整个测定过程，包括毛细管调节，大约需要30分钟。在样品浓度约为25μg/ml时，日内和日间相对标准偏差（R.S.D.）值约为7%，在样品浓度约为500μg/ml时，约为3%。血浆中的检测限为3.9μg/ml（信噪比S/N = 2）。该方法已用于药代动力学研究中患者样品中BSO非对映异构体的定量分析。