Arthur K, Hommes F A
Department of Pediatrics, New York University Medical Center, NY 10016, USA.
J Chromatogr B Biomed Appl. 1995 Nov 3;673(1):132-5. doi: 10.1016/0378-4347(95)00255-h.
A gas chromatographic-mass spectrometric method is described for the assay of propionic acid and of isovaleric acid in physiological fluids by isotope dilution. The acids are derivatized to the pentafluorobenzyl esters to decrease volatility to render them suitable for GC-MS analysis. The following reference values were found. Propionic acid: plasma 0.54 +/- 0.38 mumol/l (n = 13, range 0.03-1.38 mumol/l), urine 1.7 +/- 1.6 mumol/mmol creatinine (n = 9, range 0.1-4.9 mumol/mmol creatinine). Isovaleric acid: plasma 0.89 +/- 0.93 mumol/l (n = 10, range 0.01-3.03 mumol/l), urine 0.38 +/- 0.51 mumol/mmol creatinine (n = 10, range 0.01-1.70 mumol/mmol creatinine).
描述了一种采用同位素稀释法测定生理体液中丙酸和异戊酸的气相色谱 - 质谱法。将酸衍生化为五氟苄酯以降低挥发性，使其适合气相色谱 - 质谱分析。得到以下参考值。丙酸：血浆0.54±0.38μmol / l（n = 13，范围0.03 - 1.38μmol / l），尿液1.7±1.6μmol / mmol肌酐（n = 9，范围0.1 - 4.9μmol / mmol肌酐）。异戊酸：血浆0.89±0.93μmol / l（n = 10，范围0.01 - 3.03μmol / l），尿液0.38±0.51μmol / mmol肌酐（n = 10，范围0.01 - 1.70μmol / mmol肌酐）。
