Koumaré B, Achtman M, Bougoudogo F, Cisse M, Wang J F
Institut national de Recherche en Santé publique, Service de Bactériologie-Virologie, Bamako, Mali.
Bull World Health Organ. 1996;74(4):375-9.
The study deals with 570 strains of Neisseriaceae isolated between 1989 and 1994 in Mali: 396 of the strains were isolated from samples of cerebrospinal fluid and 174 from the throat. Serogroup C accounted for 55% of all strains. Antigenic structure was determined by ELISA, SDS-PAGE and transfer to nitrocellulose membrane for immunoblotting with monoclonal antibodies produced at the Max Planck Institute for Molecular Genetics. For serogroup A, the class 1 protein types found were P1.7 for strains isolated prior to 1994 and P1.9 for strains isolated in 1994. P1.7 is specific to clone IV-1 and P1.9 to clone III-1, which was responsible for the 1994 epidemic. All strains of serogroup C isolated from fluid CSF and most strains isolated from the throat exhibit a new type of class 1 protein which the authors have designated P1.y. P1.y is characteristic of Malian strains of serogroup C; it is rare or absent in strains from other countries (Burkina Faso, Ghana, Italy, USA). The nucleotide sequence of the gene expressing P1.y and the corresponding amino acid sequence were determined at the National Institute for Biological Standards and Control, England.
该研究涉及1989年至1994年间在马里分离出的570株奈瑟菌科细菌：其中396株从脑脊液样本中分离得到，174株从咽喉部位分离得到。血清群C占所有菌株的55%。通过酶联免疫吸附测定（ELISA）、十二烷基硫酸钠-聚丙烯酰胺凝胶电泳（SDS-PAGE）以及转移至硝酸纤维素膜上，再用马克斯·普朗克分子遗传学研究所生产的单克隆抗体进行免疫印迹法来确定抗原结构。对于血清群A，1994年之前分离出的菌株中发现的1类蛋白类型为P1.7，1994年分离出的菌株中为P1.9。P1.7是克隆IV-1特有的，P1.9是克隆III-1特有的，克隆III-1引发了1994年的疫情。从脑脊液中分离出的所有血清群C菌株以及从咽喉部位分离出的大多数菌株都呈现出一种新型的1类蛋白，作者将其命名为P1.y。P1.y是马里血清群C菌株的特征；在其他国家（布基纳法索、加纳、意大利、美国）的菌株中很少见或不存在。表达P1.y的基因的核苷酸序列以及相应的氨基酸序列是在英国国家生物标准与控制研究所测定的。
