Pejler G
Swedish University of Agricultural Sciences, Department of Veterinary Medical Chemistry, The Biomedical Center, Uppsala, Sweden.
FEBS Lett. 1996 Apr 1;383(3):170-4. doi: 10.1016/0014-5793(96)00239-6.
Protamines are polycationic proteins that are widely used for neutralisation of the anticoagulant action of heparin. However, several reports have shown adverse, mast cell-dependent reactions to protamine. The exact mechanism by which protamine causes these adverse effects is not clear. In the present study, the possibility that protamine may influence mast cell chymase function was investigated. Mast cell chymase is in vivo recovered in a macromolecular complex with heparin proteoglycan, and this interaction is essential for expression of optimal enzymatic activity. Protamine was shown to strongly reduce the activity of mast cell chymase by a mechanism that involved displacement of the chymase from heparin proteoglycan.
鱼精蛋白是多阳离子蛋白，广泛用于中和肝素的抗凝作用。然而，有几份报告显示了鱼精蛋白引起的不良的、肥大细胞依赖性反应。鱼精蛋白导致这些不良反应的确切机制尚不清楚。在本研究中，研究了鱼精蛋白可能影响肥大细胞糜酶功能的可能性。肥大细胞糜酶在体内以与肝素蛋白聚糖的大分子复合物形式回收，这种相互作用对于最佳酶活性的表达至关重要。结果表明，鱼精蛋白通过将糜酶从肝素蛋白聚糖中置换出来的机制，强烈降低肥大细胞糜酶的活性。
