Pitzer F, Dantes A, Fuchs T, Baumeister W, Amsterdam A
The Max-Planck-Institut für Biochemie, Martinsried, Germany.
FEBS Lett. 1996 Sep 23;394(1):47-50. doi: 10.1016/0014-5793(96)00920-9.
Apoptosis can be initiated in immortalized cAMP-stimulated rat ovarian granulosa cells by induction of wild-type p53 activity. Immunocytochemical studies using confocal microscopy reveal that in apoptotic, unlike in normal growing cells, the proteasomes are removed from the nucleus and accumulate within the apoptotic blebs at the periphery of the cell. In parallel, a striking reorganization of the actin cytoskeleton is observed which forms a spherical network separating the apoptotic blebs from the cytoplasmic organelles, such as mitochondria and lipid droplets which remain in the perinuclear region. The reorganization of the actin cytoskeleton as well as disappearance of proteasomes from the nucleus suggest possible function of proteasomes in apoptotic regulation.
通过诱导野生型p53活性，可在永生化的cAMP刺激的大鼠卵巢颗粒细胞中引发凋亡。使用共聚焦显微镜的免疫细胞化学研究表明，在凋亡细胞中，与正常生长细胞不同，蛋白酶体从细胞核中移除并聚集在细胞周边的凋亡小泡内。同时，观察到肌动蛋白细胞骨架发生显著重组，形成一个球形网络，将凋亡小泡与细胞质细胞器（如留在核周区域的线粒体和脂滴）分隔开。肌动蛋白细胞骨架的重组以及蛋白酶体从细胞核中的消失表明蛋白酶体在凋亡调节中可能发挥作用。
