Pforte A, Schiessler A, Kressenstein S, Gais P, Beer B, Hüssinger K, Riethmüller G, Ziegler-Heitbrock H W
Department of Internal Medicine, University of Munich, Germany.
J Clin Lab Immunol. 1995;46(3):125-35.
The intercellular adhesion molecule-1 (ICAM-1) is important in mediating intercellular contact in inflammation. Therefore, we have analyzed the expression of this molecule on alveolar macrophages (AM) and in serum of patients with sarcoidosis. Bronchoalveolar lavage (BAL) cells from 13 patients and 11 control donors were stained with an anti ICAM-1 monoclonal antibody (mAb) or an isotype control. Alkaline phosphatase was used as a detection system, followed by digital single cell image analysis. Soluble ICAM-1 in serum (ssICAM-1) was determined by enzyme linked immunoassay (Elisa). Immunocytochemistry revealed a strong increase of ICAM-1 expression on AM from sarcoidosis patients (64%) compared to healthy controls (30%). Furthermore, patients exhibited a fourfold higher antigen density. Serum levels of sICAM-1 were more than twofold increased in the patient group (805.4 micrograms/ml) compared to healthy controls (384.8 micrograms/ml). SsICAM-1 showed an inverse correlation with vital capacity (VC) and diffusing capacity (DCO). This significant correlation with impairment of two important lung function parameters suggests that ssICAM-1 might be useful in serological assessment of disease activity in sarcoidosis.
细胞间黏附分子-1（ICAM-1）在介导炎症中的细胞间接触方面很重要。因此，我们分析了该分子在结节病患者肺泡巨噬细胞（AM）及血清中的表达情况。对13例患者和11例对照供体的支气管肺泡灌洗（BAL）细胞用抗ICAM-1单克隆抗体（mAb）或同型对照进行染色。使用碱性磷酸酶作为检测系统，随后进行数字单细胞图像分析。通过酶联免疫吸附测定（ELISA）测定血清中的可溶性ICAM-1（ssICAM-1）。免疫细胞化学显示，与健康对照（30%）相比，结节病患者AM上ICAM-1表达显著增加（64%）。此外，患者的抗原密度高出四倍。与健康对照（384.8微克/毫升）相比，患者组血清sICAM-1水平增加了两倍多（805.4微克/毫升）。SsICAM-1与肺活量（VC）和弥散功能（DCO）呈负相关。与两项重要肺功能参数受损的这种显著相关性表明，ssICAM-1可能有助于结节病疾病活动的血清学评估。
