Mishima H K, Nii H, Kurokawa T, Kiuchi Y
Department of Ophthalmology, Hiroshima University School of Medicine, Japan.
Jpn J Ophthalmol. 1995;39(4):317-22.
The authors investigated the influence of the elevation of intracellular Ca2+ concentration on the production of cyclic adenosine 3',5'-monophosphate (cAMP) using cultured chick embryo ciliary epithelium (CE). We examined the effects of calcium ionophore (A23187) on cAMP production after incubation with vasoactive intestinal peptide (VIP), isoproterenol (ISO), sodium fluoride (NaF) or forskolin (FSK). A23187 had no effect on the basal cAMP level, or the NaF- and FSK-stimulated responses for cAMP level; but A23187 potentiated VIP- and ISO-stimulated cAMP responses. Calmodulin antagonist (W-7) was very effective in inhibiting the potentiating effects of A23187 on VIP and ISO-stimulated production of cAMP. Our present findings suggested that Ca2+/calmodulin may potentiate the receptor-mediated cAMP pathway through Ca2+/calmodulin-dependent protein kinase in the CE.
作者使用培养的鸡胚睫状体上皮（CE）研究了细胞内Ca2+浓度升高对环磷酸腺苷（cAMP）产生的影响。我们检测了钙离子载体（A23187）与血管活性肠肽（VIP）、异丙肾上腺素（ISO）、氟化钠（NaF）或福斯高林（FSK）孵育后对cAMP产生的影响。A23187对基础cAMP水平或NaF和FSK刺激的cAMP水平反应没有影响；但A23187增强了VIP和ISO刺激的cAMP反应。钙调蛋白拮抗剂（W-7）在抑制A23187对VIP和ISO刺激的cAMP产生的增强作用方面非常有效。我们目前的研究结果表明，Ca2+/钙调蛋白可能通过CE中的Ca2+/钙调蛋白依赖性蛋白激酶增强受体介导的cAMP途径。
