Ogawa T, Ohara K, Shimizu H, Terai T, Watanabe N
Department of Ophthalmology, Jichi Medical School, Tochigi, Japan.
Jpn J Ophthalmol. 1995;39(4):353-9.
The effects of a combined use of steroidal and nonsteroidal anti-inflammatory topical drugs on uveitis were studied. Uveitis was induced by intravenous injection of E. coli endotoxin (10 micrograms/kg) in pigmented rabbits. A solution of 0.5% indomethacin (IM) and/or 0.1% betamethasone (BM) was instilled 4 times: at 1 hour before and at 0, 2 and 4 hours after the endotoxin injection. The aqueous protein was quantified by laser flare-cell measurements hourly after the injection. The prostaglandin E2 concentration was assayed and venous leukocytes and thrombocytes were counted 5 hours after the endotoxin injection. The aqueous protein reached a peak concentration (27 mg/mL) about 4 hours after the endotoxin injection. IM, BM and a combination of IM and BM (IM + BM) inhibited the aqueous protein rise by 17, 63 and 94%, respectively. IM, BM and IM + BM almost completely inhibited the increase of aqueous prostaglandin E2. IM + BM inhibited both leukocytopenia and thrombocytopenia. IM alone had no effect on these counts, and BM alone inhibited leukocytopenia only. The data suggest that the combined use of topical IM and BM is more useful for the therapy of endotoxin-induced uveitis than each drug alone. The inhibitory effects may be synergistic, probably acting on unknown mediators which may be derived from the systemic circulation.
研究了甾体类和非甾体类抗炎局部用药联合使用对葡萄膜炎的影响。通过给有色家兔静脉注射大肠杆菌内毒素（10微克/千克）诱导葡萄膜炎。在注射内毒素前1小时以及注射后0、2和4小时，滴注4次0.5%吲哚美辛（IM）溶液和/或0.1%倍他米松（BM）溶液。注射后每小时通过激光闪光细胞测量法定量房水蛋白。在内毒素注射5小时后测定前列腺素E2浓度，并对静脉白细胞和血小板进行计数。内毒素注射后约4小时，房水蛋白达到峰值浓度（27毫克/毫升）。IM、BM以及IM与BM的组合（IM + BM）分别使房水蛋白升高抑制了17%、63%和94%。IM、BM和IM + BM几乎完全抑制了房水前列腺素E2的增加。IM + BM抑制了白细胞减少和血小板减少。单独使用IM对这些计数没有影响，单独使用BM仅抑制白细胞减少。数据表明，局部使用IM和BM联合治疗内毒素诱导的葡萄膜炎比单独使用每种药物更有效。抑制作用可能具有协同性，可能作用于可能源自体循环的未知介质。
