Goh K L, Parasakthi N, Peh S C, Ong K K
Department of Medicine, Faculty of Medicine, University of Malaya, Kuala, Lumpur.
Med J Malaysia. 1995 Sep;50(3):208-11.
Sixty-three breath samples were collected from patients who underwent a 14C-urea breath test. Following ingestion of a radiolabelled 14C-labelled urea solution, breath samples containing 14C-labelled carbon dioxide were trapped in a solution containing hyamine hydroxide. Samples were then counted in a liquid scintillation counter. Breath samples were collected at 2, 15, 20, 25 and 30 minutes following ingestion of the urea solution. The presence or absence of Helicobacter pylori (HP) infection was determined on the basis of endoscopic biopsy tests which included culture, histological examination, rapid urease test and a gram stain of a fresh tissue smear. Thirty-two HP positive and 31 HP negative samples were collected. The mean counts at 15, 20, 12 and 30 minutes time points were: 4413, 4458, 4109 and 3795 dpm respectively for the positive samples and 1275, 877, 690 and 565 dpm respectively for the negative samples. Based on a cutoff value (mean of the negative samples + 3 standard deviations) for every time point, HP positive and negative samples could be clearly differentiated giving a sensitivity and specificity of 100%. The 14C-urea breath test is a reliable and convenient diagnostic test for H. pylori.
从接受¹⁴C - 尿素呼气试验的患者中收集了63份呼气样本。摄入放射性标记的¹⁴C标记尿素溶液后，含有¹⁴C标记二氧化碳的呼气样本被捕获在含有氢氧化海胺的溶液中。然后在液体闪烁计数器中对样本进行计数。在摄入尿素溶液后的2、15、20、25和30分钟收集呼气样本。根据内镜活检试验确定是否存在幽门螺杆菌（HP）感染，这些试验包括培养、组织学检查、快速尿素酶试验和新鲜组织涂片的革兰氏染色。收集了32份HP阳性和31份HP阴性样本。阳性样本在15、20、25和30分钟时间点的平均计数分别为4413、4458、4109和3795 dpm，阴性样本分别为1275、877、690和565 dpm。基于每个时间点的临界值（阴性样本的平均值 + 3个标准差），HP阳性和阴性样本可以清楚地区分，灵敏度和特异性均为100%。¹⁴C - 尿素呼气试验是一种用于检测幽门螺杆菌的可靠且方便的诊断试验。
