Jovanović R, Erceg S, Rakić S
Zavod za transfuziju krvi, Medicinski fakultet, Novi Sad.
Med Pregl. 1996;49(7-8):301-4.
Blood component transfusion therapy is an achievement of up-to-date transfusiology and is possible due to plastic equipment, centrifuge with air conditioning and modern technology which enables splitting blood into its components. Platelet concentration is a preparation of platelets gained from one unit of the whole blood and is used in therapy of patients with thrombocytopenias and thrombocytopathies. It can be preserved, depending on plastic bags, from 1 to 5 days with rotation on 22 degrees Celsius. This paper presents two methods of platelet concentration preparation (out of thrombocytes rich plasma and buffy coat) contribution of thrombocytes in concentrations and contamination of concentration with leucocytes, as well as changes during 72 hours of concentration preservation. One hundred units of thrombocytes had an average volume 51.42 ml. Platelet concentration gained out of thrombocyte rich plasma was 0.98 x 1011/l, and 0.80 x 109/l leucocytes. The number of thrombocytes in concentrations from buffy coat was 0.82 x 101/l, and 0.10 x 109/l leucocytes. Plasma pH after preparation was 7.21, and three days of conservation later it was 6.68. During these 72 hours of preserving the concentration there has not been a significant decrease in regard to the number of thrombocytes.
血液成分输血疗法是现代输血学的一项成果，借助塑料设备、带空调的离心机以及能将血液分离成其各种成分的现代技术才得以实现。血小板浓缩液是从一个单位全血中获取的血小板制剂，用于治疗血小板减少症和血小板病患者。根据塑料袋的类型，它可在22摄氏度旋转保存1至5天。本文介绍了两种制备血小板浓缩液的方法（从富含血小板血浆和 Buffy 层制备）、血小板在浓缩液中的含量、浓缩液被白细胞污染的情况以及浓缩液保存72小时期间的变化。100个单位的血小板平均体积为51.42毫升。从富含血小板血浆中获得的血小板浓缩液血小板含量为0.98×10¹¹/升，白细胞含量为0.80×10⁹/升。从 Buffy 层获得的浓缩液中血小板数量为0.82×10¹¹/升，白细胞数量为0.10×10⁹/升。制备后血浆pH值为7.21，保存三天后为6.68。在这72小时的浓缩液保存期间，血小板数量没有显著减少。
