Simone G, Mangia A, Petroni S, Marzullo F, Paradiso A, Schittulli F
Unità Operative di Anatomia Patologica e Citodiagnostica, IRCCS Oncologico, Bari.
Pathologica. 1996 Apr;88(2):111-6.
In order to observe receptor expression (ER and PgR) in human breast cancer cells during the S-phase of the cellular cycle, we evaluated 21 tumoral samples using a double labeling immunocytochemical-autoradiographic assay. In 16 of these we have verified that expression of ER and PgR is not influenced by incubation with 3H-Thymidine for 60 minutes. The results also demonstrated that receptor expression during the S-phase is more common in postmenopausal tumors and is principally dependent on percentages of ER+ (p < 0.01) and PgR+ (p < 0.01) cells; moreover, proliferative activity occurs almost exclusively in ER- (p = 0.003) and Pgr- (p = 0.001) cellular sub populations and the presence of PgR, but not of ER, is related to arrest of DNA synthesis (p = 0.02).
为了观察细胞周期S期人乳腺癌细胞中的受体表达（雌激素受体和孕激素受体），我们使用双标记免疫细胞化学 - 放射自显影测定法评估了21个肿瘤样本。在其中16个样本中，我们证实雌激素受体和孕激素受体的表达不受3H - 胸腺嘧啶孵育60分钟的影响。结果还表明，S期的受体表达在绝经后肿瘤中更为常见，并且主要取决于雌激素受体阳性（p <0.01）和孕激素受体阳性（p <0.01）细胞的百分比；此外，增殖活性几乎仅发生在雌激素受体阴性（p = 0.003）和孕激素受体阴性（p = 0.001）细胞亚群中，并且孕激素受体而非雌激素受体的存在与DNA合成停滞有关（p = 0.02）。
