Ferraro A, Eufemi M, Cervoni L, Altieri F, Turano C
Dipartimento di Scienze Biochimiche "Alessandro Rossi Fanelli' e Centro di Biologia Molecolare del CNR, Universitá la Sapienźa, Roma, Italy.
Physiol Chem Phys Med NMR. 1995;27(4):313-20.
DNA in cell nuclei is organized in large loops, which are formed by the binding of DNA to proteins present in a nuclear structure called matrix or scaffold. There is ample evidence that these interactions have not only a structural role, but also a functional one. Studies of these interactions have so far been carried out mainly in vitro. Their relevance, therefore, for the in vivo situation is not proven. We have analyzed the DNA-protein interactions directly in the intact nucleus by means of cross-linking reactions. Cross-linking by UV irradiation and by cis-diamine dichloro platinum (II) have been performed on nuclei from chicken liver and compared. The platinum complex has been found to be more efficient. The proteins complexed to DNA have been isolated and analyzed, and have been found to be enriched in species present in the nuclear scaffold, independently from its method of preparation.
细胞核中的DNA以大环形式组织，这些大环是由DNA与存在于称为基质或支架的核结构中的蛋白质结合形成的。有充分证据表明，这些相互作用不仅具有结构作用，还具有功能作用。迄今为止，对这些相互作用的研究主要在体外进行。因此，它们在体内情况下的相关性尚未得到证实。我们通过交联反应直接分析了完整细胞核中的DNA-蛋白质相互作用。对鸡肝细胞核进行了紫外线照射和顺二胺二氯铂（II）交联，并进行了比较。已发现铂配合物更有效。与DNA复合的蛋白质已被分离和分析，并且发现它们富含核支架中存在的物种，与制备方法无关。
