Kristensen J S, Hokland P
Arhus Amtssygehus, medicinsk-haematologisk afdeling.
Ugeskr Laeger. 1996 Oct 21;158(43):6098-102.
Immunological phenotyping of mononuclear cell suspensions from bone marrow and/or peripheral blood from 2341 patients (6140 examinations) suspected of malignant haematological diseases was performed during a 10-year period. The cells were labelled with a panel of monoclonal antibodies and subjected to flowcytometry. The results showed a clear distinction between acute lymphoblastic leukaemia and acute myeloid leukaemia, and a new group of acute hybrid leukaemia (3.3% of all acute leukaemia cases) was established. During the 10-year period immunological phenotyping has changed from being a research method to a widely employed method for cell characterisation early in the course of malignant haematological diseases.
在10年期间，对2341例疑似恶性血液病患者（共进行了6140次检查）的骨髓和/或外周血单核细胞悬液进行了免疫表型分析。细胞用一组单克隆抗体标记后进行流式细胞术检测。结果显示急性淋巴细胞白血病和急性髓细胞白血病之间有明显区别，并确定了一组新的急性混合性白血病（占所有急性白血病病例的3.3%）。在这10年期间，免疫表型分析已从一种研究方法转变为在恶性血液病病程早期广泛应用的细胞特征鉴定方法。
