Heuss D
Department of Neurology, Friedrich Alexander University of Erlangen-Nuremberg, Germany.
Acta Neuropathol. 1996;91(4):409-15. doi: 10.1007/s004010050443.
Phosphoprotein B-50, also termed neuromodulin or growth-associated protein GAP43, is a membrane-bound molecule expressed in neurons. It is particularly abundant during periods of axonal outgrowth in development and regeneration of the central and peripheral nervous systems. Recently it was reported that B-50 plays a role in the growth morphology of regenerating muscle fibers. Moreover, in vitro studies have demonstrated that the expression of B-50 in pheochromocytoma PC12 cells can be stimulated by the nerve growth factor (NGF). Expression of the low-affinity nerve growth factor receptor (LNGFR) during muscle regeneration has also been reported. Here, we studied the expression of NGF, LNGFR and B-50 in myopathy. To investigate the state of regeneration, we examined serial sections stained to demonstrate neural cell adhesion molecule and desmin. Light microscopy showed that muscle fiber regeneration in idiopathic inflammatory myopathy corresponds closely to NGF, LNGFR and B-50 immunoreactivity. The coexpression of phosphoprotein B-50, NGF and LNGFR in regenerating muscle fiber corroborates the assumption that in muscle there is a trophic pathway concerning phosphorylation or de novo synthesis of B-50 by the NGF via the LNGFR. In conclusion, a simultaneous expression of NGF, LNGFR and B-50 in muscle plays a role in the growth morphology of regenerating muscle fibers.
磷蛋白B - 50，也被称为神经调节蛋白或生长相关蛋白GAP43，是一种在神经元中表达的膜结合分子。在中枢和外周神经系统发育及再生过程中的轴突生长阶段，它尤为丰富。最近有报道称，B - 50在再生肌纤维的生长形态中发挥作用。此外，体外研究表明，神经生长因子（NGF）可刺激嗜铬细胞瘤PC12细胞中B - 50的表达。也有报道称在肌肉再生过程中低亲和力神经生长因子受体（LNGFR）的表达情况。在此，我们研究了NGF、LNGFR和B - 50在肌病中的表达。为了研究再生状态，我们检查了经染色以显示神经细胞黏附分子和结蛋白的连续切片。光学显微镜检查显示，特发性炎症性肌病中的肌纤维再生与NGF、LNGFR和B - 50免疫反应性密切相关。再生肌纤维中磷蛋白B - 50、NGF和LNGFR的共表达证实了这样一种假设，即肌肉中存在一条通过LNGFR由NGF介导的关于B - 50磷酸化或从头合成的营养途径。总之，肌肉中NGF、LNGFR和B - 50的同时表达在再生肌纤维的生长形态中发挥作用。
