Breyer M D, Davis L, Jacobson H R, Breyer R M
Department of Pharmacology, Veterans Affairs Medical Center, Nashville, Tennessee 37232-2372, USA.
Am J Physiol. 1996 May;270(5 Pt 2):F912-8. doi: 10.1152/ajprenal.1996.270.5.F912.
Four prostaglandin E2 (PGE2) receptors designated EP1, EP2, EP3, and EP4 have been pharmacologically identified, cloned, and sequenced. The present studies determined the intrarenal distribution of these EP-receptor subtypes in human kidney using in situ hybridization with riboprobes for the human EP receptors. mRNA for the phosphatidylinositol hydrolysis-coupled EP receptor was highly expressed in cortical, outer medullary, and inner medullary collecting duct. RNA for the Gi-coupled EP3 receptor was primarily expressed in the cortical and outer medullary collecting duct, as well as in the medullary thick ascending limb; however, it was absent from the inner medullary collecting duct. Expression of mRNA for EP1 and EP3 in connecting segment could not be excluded. There was no expression of the GS-coupled EP2 receptor mRNA detected in human kidney by in situ hybridization; however, mRNA for the GS-coupled EP4 receptor was highly expressed in the glomerulus. These studies demonstrate distinct regions of intrarenal expression for the different EP receptors and suggest that each receptor subtype may modulate different aspects of renal function in humans.
已通过药理学方法鉴定、克隆并测序了四种前列腺素E2（PGE2）受体，分别命名为EP1、EP2、EP3和EP4。本研究采用与人EP受体的核糖核酸探针进行原位杂交的方法，确定了这些EP受体亚型在人肾内的分布。与磷脂酰肌醇水解偶联的EP受体的mRNA在皮质、外髓和内髓集合管中高度表达。与Gi偶联的EP3受体的RNA主要表达于皮质和外髓集合管以及髓质厚壁升支；然而，在内髓集合管中未检测到。不能排除连接段中EP1和EP3的mRNA表达。原位杂交未在人肾中检测到与GS偶联的EP2受体mRNA的表达；然而，与GS偶联的EP4受体的mRNA在肾小球中高度表达。这些研究证明了不同EP受体在肾内的不同表达区域，并表明每种受体亚型可能调节人类肾功能的不同方面。
