Mosier D A, Simons K R, Vestweber J G
Department of Pathology, College of Veterinary Medicine, Kansas State University, Manhattan 66506, USA.
Am J Vet Res. 1995 Oct;56(10):1317-21.
Four colostrum-deprived calves each were immunized passively with antisera to whole Pasteurella haemolytica, leukotoxin-containing supernatants of P haemolytica, P haemolytica lipopolysaccharide, or newborn calf serum. Calves were challenge exposed intrabronchially with 5 x 10(9) P haemolytica, and 24 hours later, the resulting lesions were evaluated. The greatest protection against challenge exposure was provided by the antiserum to whole P haemolytica (lesion score = 6.3), whereas newborn calf serum provided the least protection (lesion score = 28.3). Calves that received antiserum to P haemolytica supernatants were moderately protected (lesion score = 16.3), and the antiserum to lipopolysaccharide provided minimal protection (lesion score = 21.8). Antibodies that were unique to whole P haemolytica antiserum and produced dense bands on immunoblots were detected to antigens at 66, 50, and 30 kd. Antibodies in the supernatant preparation that produced prominent bands reacted to antigens between 100 and 90 kd. Collectively, antibodies to these antigens may be responsible for enhancing resistance to experimentally induced pneumonic pasteurellosis. Antibodies to antigens in P haemolytica lipopolysaccharide provided little to no protection.
给4头未哺初乳的犊牛分别被动免疫抗全溶血巴斯德氏菌血清、含溶血巴斯德氏菌白细胞毒素的上清液、溶血巴斯德氏菌脂多糖或新生犊牛血清。通过支气管内接种5×10⁹ 溶血巴斯德氏菌对犊牛进行攻毒，24小时后评估所产生的病变。抗全溶血巴斯德氏菌血清对攻毒的保护作用最强（病变评分=6.3），而新生犊牛血清提供的保护作用最小（病变评分=28.3）。接受抗溶血巴斯德氏菌上清液血清的犊牛受到中度保护（病变评分=16.3），抗脂多糖血清提供的保护作用最小（病变评分=21.8）。在免疫印迹上产生致密条带的全溶血巴斯德氏菌抗血清特有的抗体，可与66、50和30kd的抗原发生反应。上清液制剂中产生明显条带的抗体与100至90kd之间的抗原发生反应。总的来说，针对这些抗原的抗体可能有助于增强对实验性诱发的肺炎巴氏杆菌病的抵抗力。溶血巴斯德氏菌脂多糖中抗原的抗体几乎没有提供保护作用。
