Coleman G, Abbas-Ali B
Infect Immun. 1977 Aug;17(2):278-81. doi: 10.1128/iai.17.2.278-281.1977.
A comparison has been made of total exoprotein formation by Staphylococcus aureus (Wood 46) in a tryptone soya broth (TSB) medium and a casein hydrolysate-yeast extract-containing (CCY) medium in which exponential growth occurred with doubling times of 3.0 and 1.3 h, respectively. It was found that the differential rate of exoprotein formation was biphasic in each case, increasing after the cessation of exponential growth by a factor of 4 in TSB and 7 in CCY medium. Although this relative change was greater in CCY medium, the maximum value of the differential rate was less than 40% of that achieved in TSB medium. It was further shown that throughout the growth cycle, and in both cultures, alpha-toxin accounted for the same proportion of total extracellular protein.
对金黄色葡萄球菌（伍德46株）在胰蛋白胨大豆肉汤（TSB）培养基和含酪蛋白水解物 - 酵母提取物（CCY）的培养基中总外蛋白形成情况进行了比较。在这两种培养基中，金黄色葡萄球菌呈指数生长，其倍增时间分别为3.0小时和1.3小时。结果发现，每种情况下外蛋白形成的微分速率都是双相的，在指数生长停止后，TSB培养基中增加了4倍，CCY培养基中增加了7倍。虽然这种相对变化在CCY培养基中更大，但微分速率的最大值不到TSB培养基中所达到值的40%。进一步表明，在整个生长周期以及两种培养物中，α - 毒素在总细胞外蛋白中所占比例相同。
