Gross S, Gase K, Malke H
Institute for Molecular Biology, Jena University, Winzerlaer Strasse 10, D-07745 Jena, Germany.
Arch Microbiol. 1996 Aug;166(2):116-21. doi: 10.1007/s002030050364.
DNA sequences upstream of the core promoter region of the streptokinase gene (skc) from Streptococcus equisimilis H46A increase skc transcription more than tenfold in vivo. This promoter upstream region contains a segment of intrinsically bent DNA, the precise location of which was determined experimentally by circular permutation analysis and theoretically by computer prediction. Electrophoretic analysis of circularly permuted upstream DNA fragments placed the bend center approximately at position -100 with respect to the major transcription initiation site of skc. This position was in excellent agreement with the center of maximum curvature predicted theoretically. Knowledge of the precise location of the bend center will be useful for future studies of the possible effect of DNA bending on skc transcription.
来自类马链球菌H46A的链激酶基因（skc）核心启动子区域上游的DNA序列在体内可使skc转录增加十多倍。该启动子上游区域包含一段固有弯曲的DNA，其精确位置通过环形置换分析实验确定，并通过计算机预测从理论上确定。对环形置换的上游DNA片段进行电泳分析，将弯曲中心定位在相对于skc主要转录起始位点约-100的位置。该位置与理论预测的最大曲率中心高度吻合。弯曲中心精确位置的信息将有助于未来研究DNA弯曲对skc转录可能产生的影响。
