Rauen T, Rothstein J D, Wässle H
Max-Planck-Institut für Hirnforschung, Abteilung Neuroanatomie, Deutschordenstrasse 46, D-60528 Frankfurt/M., Germany.
Cell Tissue Res. 1996 Dec;286(3):325-36. doi: 10.1007/s004410050702.
The immunocytochemical distribution of the three excitatory amino acid transporter subtypes GLT-1, GLAST-1 and EAAC-1 was studied in the rat retina using antibodies raised against synthetic peptides corresponding to the C-terminus of each transporter subtype (Rothstein et al. 1994). A comparative immunoblot analysis of rat cortex, cerebellum and retina membrane proteins suggested the following rank order of glutamate transporter subtype expression in retina: GLAST-1>/=EAAC-1>GLT-1. GLAST-1 immunoreactivity was seen in Müller cells and astrocytes. EAAC-1 was found in horizontal cells, in amacrine and displaced amacrine cells, and in ganglion cells. A minority of bipolar cells also expressed EAAC-1. GLT-1 was preferentially expressed by different types of bipolar cells; however, it was also found in some amacrine cells. The functional role of this differential distribution of glutamate transporters in the retina is discussed.
利用针对对应于每种转运体亚型C末端的合成肽产生的抗体，在大鼠视网膜中研究了三种兴奋性氨基酸转运体亚型GLT-1、GLAST-1和EAAC-1的免疫细胞化学分布（Rothstein等人，1994年）。对大鼠皮质、小脑和视网膜膜蛋白的比较免疫印迹分析表明，视网膜中谷氨酸转运体亚型表达的顺序如下：GLAST-1≥EAAC-1＞GLT-1。在穆勒细胞和星形胶质细胞中可见GLAST-1免疫反应性。在水平细胞、无长突细胞和移位无长突细胞以及神经节细胞中发现了EAAC-1。少数双极细胞也表达EAAC-1。GLT-1优先由不同类型的双极细胞表达；然而，在一些无长突细胞中也发现了它。本文讨论了谷氨酸转运体在视网膜中这种差异分布的功能作用。
