Pietz K, Odin P, Funa K, Lindvall O
Restorative Neurology Unit, Department of Neurology, University Hospital, Sweden.
Neurosci Lett. 1996 Feb 2;204(1-2):101-4. doi: 10.1016/0304-3940(96)12326-0.
The effects of platelet-derived growth factor (PDGF)-AA and -BB on 6-hydroxydopamine (6-OHDA)-induced degeneration of dopaminergic (DA) neurons were studied in cultures of dissociated fetal rat mesencephalon. The 6-OHDA lesion (0.3% for 4 h) caused a selective 75% loss of tyrosine hydroxylase (TH)-positive DA neurons. The neuronal death was significantly decreased (to about 25%) by addition of 1, 10 or 30 (but not 0.1) ng/ml of PDGF-BB 24 h prior to the insult. In contrast, treatment with PDGF-AA (30 ng/ml) for 24 h before the lesion or addition of PDGF-BB (30 ng/ml) simultaneously with or 1 h after 6-OHDA did not promote the survival of TH-positive neurons. We conclude that PDGF-BB can counteract 6-OHDA-induced degeneration of mesencephalic DA neurons.
在离体培养的胎鼠中脑，研究了血小板源性生长因子（PDGF）-AA和-BB对6-羟基多巴胺（6-OHDA）诱导的多巴胺能（DA）神经元变性的影响。6-OHDA损伤（0.3%，4小时）导致酪氨酸羟化酶（TH）阳性DA神经元选择性丧失75%。在损伤前24小时加入1、10或30（而非0.1）ng/ml的PDGF-BB，可使神经元死亡显著减少（降至约25%）。相比之下，在损伤前用PDGF-AA（30 ng/ml）处理24小时，或在6-OHDA处理的同时或之后1小时加入PDGF-BB（30 ng/ml），均不能促进TH阳性神经元的存活。我们得出结论，PDGF-BB可以对抗6-OHDA诱导的中脑DA神经元变性。
