Onozuka M, Watanabe K
Department of Anatomy, Gifu University School of Medicine, Japan.
Neurosci Lett. 1996 Feb 2;204(1-2):105-8. doi: 10.1016/0304-3940(96)12327-2.
Using the voltage-clamp technique combined with pressure injection, we examined the inhibitory action of glutamate (Glu) in identified snail neurons. Calimidazolium and N-(6-aminohexyl)-5-chloro-1-naphthalenesulfonamide (W-7), calmodulin inhibitors, enhanced a slow outward current (Glu) current induced by Glu in a dose-dependent manner. This Glu current was suppressed by intracellular injection of calmodulin. However, no significant change in the Glu current was observed when either CaCl2 or EGTA was intracellularly applied. These results suggest a novel calmodulin-mediated process, through which Glu induces an outward current.
运用电压钳技术并结合压力注射，我们研究了谷氨酸（Glu）在已鉴定的蜗牛神经元中的抑制作用。钙调素抑制剂氯咪达唑和N-(6-氨基己基)-5-氯-1-萘磺酰胺（W-7）以剂量依赖的方式增强了由Glu诱导的缓慢外向电流（Glu电流）。这种Glu电流可被细胞内注射钙调素所抑制。然而，当细胞内注入CaCl2或EGTA时，未观察到Glu电流有显著变化。这些结果提示了一种新的钙调素介导的过程，通过该过程Glu可诱导外向电流。
