Yamamoto M, Sobue G, Yamamoto K, Terao S, Mitsuma T
Department of Neurology, Nagoya University School of Medicine, Japan.
Neurosci Lett. 1996 Feb 2;204(1-2):117-20. doi: 10.1016/0304-3940(96)12342-9.
The steady-state expression levels of glial cell line-derived growth factor (GDNF) mRNA was studied in the post mortem spinal cord and muscle of patients with amyotrophic lateral sclerosis (ALS), by reverse transcription followed by polymerase chain reaction (RT-PCR). GDNF mRNA levels in the lumbar cord were significantly increased in ALS as compared with controls. On the other hand, GDNF mRNA levels were significantly lower in the muscle in ALS patients. The increase of GDNF mRNA in the spinal cord mostly reflected an increase in the anterior horn, anterior and lateral columns, where the pathological involvement was severe. In the posterior column and posterior horn with less pathological involvement, the increase was less. These results suggest that GDNF mRNA levels are inversely regulated between the spinal cord and muscle in ALS, increased in the spinal cord and lowered in the muscle, in correlation with the pathological severity.
通过逆转录聚合酶链反应（RT-PCR），对肌萎缩侧索硬化症（ALS）患者死后的脊髓和肌肉中胶质细胞系衍生神经营养因子（GDNF）mRNA的稳态表达水平进行了研究。与对照组相比，ALS患者腰段脊髓中的GDNF mRNA水平显著升高。另一方面，ALS患者肌肉中的GDNF mRNA水平显著降低。脊髓中GDNF mRNA的增加主要反映在前角、前柱和侧柱中增加，这些部位病理累及严重。在后柱和后角，病理累及较少，增加也较少。这些结果表明，在ALS中，脊髓和肌肉之间GDNF mRNA水平呈反向调节，脊髓中升高而肌肉中降低，与病理严重程度相关。
