Hecht C, Geldermann H
Department of Animal Breeding, Hohenheim University, Stuttgart, Germany.
Anim Genet. 1996 Oct;27(5):329-32.
In order to identify DNA variants potentially involved in the regulation of the expression of the bovine growth hormone gene, the 5'-flanking, the 5'-untranslated and the signal peptide coding regions of 15 cows (four German Friesian, two German Simmental, two Jersey, two Galloway, two Scottish Highland, two Charolais and one Ceylon Dwarf Zebu) were analysed. For each animal a region of about 1.2 kb was amplified by polymerase chain reaction (PCR) and sequenced. Six sites of variable nucleotides were identified in the 5'-flanking region of the gene and one in the intron I. Some of these variable sites are also potential binding sites for trans-acting factors (CAAT/enhancer binding protein (C/EBP), polyoma virus enhancer A binding protein 3 (PEA3), thyroid hormone response element (TRE)) and therefore possibly involved in the expression of the growth hormone gene. PCR amplification of specific alleles (PASA) tests for two of the sites (G2, G3) were established and the genotypes for these variants were analysed in 190 cows. The frequencies of the C alleles of the sites G2 and G3 were 0.63 and 0.76, respectively.
为了鉴定可能参与牛生长激素基因表达调控的DNA变异体，对15头奶牛（4头德国荷斯坦牛、2头德国西门塔尔牛、2头泽西牛、2头盖洛威牛、2头苏格兰高地牛、2头夏洛来牛和1头锡兰矮种瘤牛）的5'侧翼区、5'非翻译区和信号肽编码区进行了分析。对每头动物，通过聚合酶链反应（PCR）扩增约1.2 kb的区域并进行测序。在该基因的5'侧翼区鉴定出6个可变核苷酸位点，在内含子I中鉴定出1个。这些可变位点中的一些也是反式作用因子（CAAT/增强子结合蛋白（C/EBP）、多瘤病毒增强子A结合蛋白3（PEA3）、甲状腺激素反应元件（TRE））的潜在结合位点，因此可能参与生长激素基因的表达。建立了针对其中两个位点（G2、G3）的特异性等位基因PCR扩增（PASA）检测方法，并对190头奶牛的这些变异体基因型进行了分析。位点G2和G3的C等位基因频率分别为0.63和0.76。
