Quiñonez M, Romero P J, Rojas L
Instituto de Biología Experimental, Facultad de Ciencias, Universidad Central de Venezuela, Caracas, Venezuela.
Brain Res. 1996 Oct 21;737(1-2):327-30. doi: 10.1016/0006-8993(96)00950-x.
Miniature end-plate currents (MEPCS), from synaptic spots on the caudal muscles of Bufo marinus tadpoles, were analyzed in both pre- and postsynaptic domains, when protein kinase A (PKA) activity modificators were used. Sp-cAMPS diasteromer induced an increase in MEPC frequency, which was completely reversed by Rp-cAMPS. However, changes in the decay time of MEPCS were not detected. Dibutyryl-cAMP produced a similar presynaptic action, but its postsynaptic action was similar to butyrate. Presynaptic effect of forskolin (FSK), if any, is masked by the increase of MEPC frequency produced by dimethylsulfoxide (DMSO), the solvent used.
当使用蛋白激酶A（PKA）活性调节剂时，对海蟾蜍蝌蚪尾部肌肉突触位点的微小终板电流（MEPCs）在突触前和突触后区域进行了分析。Sp-cAMPS非对映体诱导MEPC频率增加，这被Rp-cAMPS完全逆转。然而，未检测到MEPCs衰减时间的变化。二丁酰环磷腺苷产生了类似的突触前作用，但其突触后作用与丁酸盐相似。福斯高林（FSK）的突触前效应（如果有的话）被所用溶剂二甲基亚砜（DMSO）产生的MEPC频率增加所掩盖。
