Coursin D B, Cihla H P, Sempf J, Oberley T D, Oberley L W
Department of Anesthesiology, University of Wisconsin Clinical Science Center, Madison, USA.
Histol Histopathol. 1996 Oct;11(4):851-60.
Samples of normal human lung and six major types of human lung carcinomas were immunostained for antioxidant enzymes (manganese and copper, zinc superoxide dismutases, catalase, and glutathione peroxidase) and six isoenzymes of glutathione S-transferase staining was generally low in tumor cells compared with the high level of staining noted in respiratory epithelium. A notable exception was heterogeneity in immunostaining for manganese superoxide dismutase in lung adenocarcinoma, which showed both positive and negative cells in the same tumor. Tumor stromal cells (fibroblast-appearing cells) often showed strong immunostaining for manganese superoxide dismutase, while stromal cells were negative for other antioxidant and glutathione S-transferase enzymes. None of the carcinomas studied had significant levels of catalase or glutathione peroxidase; this finding has potential clinical relevance since it indicates that these tumors cannot detoxify hydrogen peroxide. The low levels of antioxidant and glutathione S-transferase enzymes in tumor cells is consistent with the hypothesis that these enzymes are markers of cell differentiation.
对正常人肺组织样本以及六种主要类型的人肺癌组织进行免疫染色，检测抗氧化酶（锰超氧化物歧化酶、铜锌超氧化物歧化酶、过氧化氢酶和谷胱甘肽过氧化物酶）以及谷胱甘肽S -转移酶的六种同工酶。与呼吸道上皮细胞中明显的高染色水平相比，肿瘤细胞中的染色通常较低。一个显著的例外是肺腺癌中锰超氧化物歧化酶免疫染色的异质性，在同一肿瘤中既有阳性细胞也有阴性细胞。肿瘤基质细胞（成纤维细胞样细胞）通常对锰超氧化物歧化酶显示出强免疫染色，而基质细胞对其他抗氧化酶和谷胱甘肽S -转移酶呈阴性。所研究的癌组织中均没有显著水平的过氧化氢酶或谷胱甘肽过氧化物酶；这一发现具有潜在的临床意义，因为它表明这些肿瘤无法解毒过氧化氢。肿瘤细胞中抗氧化酶和谷胱甘肽S -转移酶水平较低与这些酶是细胞分化标志物的假设一致。
