Scannell M P, Yeh S R, Falvey D E
Department of Chemistry and Biochemistry, University of Maryland, College Park 20742, USA.
Photochem Photobiol. 1996 Nov;64(5):764-8. doi: 10.1111/j.1751-1097.1996.tb01832.x.
The enzyme DNA photolyase mediates the repair of pyrimidine dimers. This repair step, a net retro [2 + 2] reaction, proceeds through either the cation or anion radical of the pyrimidine dimer. In order to understand how electron transfer makes the repair process possible, its energetics have been examined by photothermal beam deflection calorimetry, fluorescence quenching and quantum yield studies. The enthalpy for the cleavage reaction of cis-syn 1,3-dimethylthymine dimer itself was found to be -19 kcal/mol. In addition, from the redox potentials, the enthalpies for the cleavage reactions of the dimer cation radical and the anion radical were determined to be -19 kcal/mol and -28 kcal/mol, respectively.
