一种连接酵母人工染色体的方法。
A method for linking yeast artificial chromosomes.
作者信息
Larin Z, Taylor S S, Tyler-Smith C
机构信息
Institute of Molecular Medicine, University of Oxford, John Radcliffe Hospital, Headington, UK.
出版信息
Nucleic Acids Res. 1996 Nov 1;24(21):4192-6. doi: 10.1093/nar/24.21.4192.
Abstract
A method for linking any standard yeast artificial chromosomes (YAC) is described. YACs are introduced into the same cell and joined by mitotic recombination between the vector arms and the homologous sequence in a linking vector; several YACs can be recombined sequentially. The linking vectors also contain the beta-galactosidase gene as an expression reporter in mammalian cells.
摘要
描述了一种连接任何标准酵母人工染色体（YAC）的方法。将YAC引入同一细胞，并通过载体臂与连接载体中的同源序列之间的有丝分裂重组进行连接；几个YAC可以依次重组。连接载体还包含β-半乳糖苷酶基因作为哺乳动物细胞中的表达报告基因。
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