Melkonyan H, Sorg C, Klempt M
Institute of Experimental Dermatology, University of Münster, Germany.
Nucleic Acids Res. 1996 Nov 1;24(21):4356-7. doi: 10.1093/nar/24.21.4356.
Electroporation is one of the most common methods used transform mammalian cells with plasmids. This method is versatile and can be adapted to meet the requirements of many cell lines. However, sometimes the efficiency of this method is low. We demonstrate that dimethyl sulfoxide (DMSO) facilitated a better DNA uptake in four different cell lines (HL60, TR146, Cos-7 and L132). The cells were electroporated with a beta-Gal expression plasmid in a medium containing DMSO (1.25%) during, and for 24 h after the pulse. In all these cells a dramatic (up to 8-fold) increase in transfection efficiency occurred after this treatment. This method opens up the possibility of using electroporation even in cells which are difficult to transfect.
电穿孔是用质粒转化哺乳动物细胞最常用的方法之一。该方法用途广泛，可根据多种细胞系的要求进行调整。然而，有时这种方法的效率较低。我们证明，二甲基亚砜（DMSO）能促进四种不同细胞系（HL60、TR146、Cos-7和L132）更好地摄取DNA。在含有DMSO（1.25%）的培养基中，于脉冲期间及脉冲后24小时，用β-半乳糖苷酶表达质粒对细胞进行电穿孔。经过这种处理后，所有这些细胞的转染效率都显著提高（高达8倍）。这种方法为即使在难以转染的细胞中使用电穿孔开辟了可能性。
