Takita-Sonoda Y, Tsuji M, Kamboj K, Nussenzweig R S, Clavijo P, Zavala F
Department of Medical and Molecular Parasitology, New York University School of Medicine, New York 10010, USA.
Exp Parasitol. 1996 Nov;84(2):223-30. doi: 10.1006/expr.1996.0108.
In this study we characterized the CD4+ T cell response directed against two distinct epitopes located in the circumsporozoite (CS) protein of Plasmodium yoelii. The immunization of mice with P. yoelii sporozoites induced CD4+ T cells which were mostly directed against one of these peptides, Py-1, previously reported to contain a CD4+ epitope. The CD4+ T cells directed against this immunodominant epitope were mostly of the Th-1 type. Another newly identified peptide, AS44, induced a specific CD4+ T cell response, which was mainly detectable after immunization with the corresponding peptide. Several CD4+ T cell clones, recognizing this epitope, were generated and their lymphokine expression was characterized, as well as their surface markers and their anti-parasite activity in vivo. It was noteworthy that some of these CD4+ T cell clones, which recognize this cryptic epitope and were of different Th subtypes, were shown to have a strong inhibitory effect on the development of liver stages of malaria parasites.
在本研究中，我们对针对约氏疟原虫环子孢子（CS）蛋白中两个不同表位的CD4 + T细胞应答进行了表征。用约氏疟原虫子孢子免疫小鼠可诱导CD4 + T细胞，这些细胞大多针对其中一种肽Py-1，此前报道该肽含有一个CD4 +表位。针对这一免疫显性表位的CD4 + T细胞大多为Th-1型。另一种新鉴定的肽AS44可诱导特异性CD4 + T细胞应答，在用相应肽免疫后主要可检测到该应答。产生了几个识别该表位的CD4 + T细胞克隆，并对它们的淋巴因子表达、表面标志物以及它们在体内的抗寄生虫活性进行了表征。值得注意的是，这些识别该隐蔽表位且属于不同Th亚型的CD4 + T细胞克隆中的一些，对疟原虫肝期发育具有强烈的抑制作用。
