Ouderkirk L A
J Assoc Off Anal Chem. 1977 Sep;60(5):1116-8.
A paper disk method based on a procedure described by the International Dairy Federation is presented for the detection of 4 beta-lactam antibiotics (penicillin G, ampicillin, cephapirin, and cloxacillin) in whole milk. Bacillus stearothermophilus var. calidolactis, prepared as a stable spore suspension, was used as the test organism. Levels of 0.005 unit penicillin G/ml, 0.005 microgram ampicillin or cephapirin/ml, and 0.05 microgram cloxacillin/ml were readily detected in whole milk. Results were produced in 3-4 hr. The method offers several advantages, including greater simplicity, sensitivity, and rapidity, over methods now commonly used to detect residual levels of these drugs in milk.
本文介绍了一种基于国际乳品联合会所述程序的纸片法，用于检测全脂牛奶中的4种β-内酰胺抗生素（青霉素G、氨苄青霉素、头孢匹林和氯唑西林）。嗜热脂肪芽孢杆菌变种嗜热栖热菌制备成稳定的孢子悬浮液，用作测试微生物。全脂牛奶中0.005单位青霉素G/毫升、0.005微克氨苄青霉素或头孢匹林/毫升以及0.05微克氯唑西林/毫升的水平很容易被检测到。结果在3-4小时内得出。与目前常用的检测牛奶中这些药物残留水平的方法相比，该方法具有几个优点，包括更简单、灵敏和快速。
