Masuda T, Shimura S, Maruyama N, Nara M, Shirato K
First Department of Internal Medicine, Tohoku University School of Medicine, Sendai, Japan.
Respiration. 1996;63(6):358-62. doi: 10.1159/000196577.
We examined the effects of histamine, tumor necrosis factor-alpha (TNF alpha), interferon-gamma (IFN gamma), indomethacin and dexamethasone on the adherence of eosinophils to airway epithelial cells. Epithelial cells were isolated from guinea pig trachea through enzymatic digestion, and cultured until they showed confluence. Eosinophils were taken by peritoneal lavage from guinea pigs sensitized with horse serum and purified. The adhesion of eosinophils to cultured epithelial cells was examined using 51Cr. The percentage adhesion was obtained by dividing counts per minute of lysed eosinophils adhered to epithelial cells by suspended eosinophils. Histamine significantly increased the adhesion of eosinophils to epithelial cells, while TNF alpha or IFN gamma did not significantly alter the adhesion. Indomethacin significantly inhibited the adhesion of eosinophils to epithelial cells in both nontreated and histamine-induced conditions. Dexamethasone also reduced adhesion, although it did not reach statistical significance. These findings indicate that histamine induced eosinophil adherence to airway epithelial cells, suggesting a role of endogenous prostaglandins in adherence.
我们研究了组胺、肿瘤坏死因子-α（TNFα）、干扰素-γ（IFNγ）、吲哚美辛和地塞米松对嗜酸性粒细胞与气道上皮细胞黏附的影响。通过酶消化从豚鼠气管分离上皮细胞，并培养至汇合。用马血清致敏的豚鼠经腹腔灌洗获取嗜酸性粒细胞并进行纯化。使用51Cr检测嗜酸性粒细胞与培养的上皮细胞的黏附情况。黏附百分比通过将黏附于上皮细胞的裂解嗜酸性粒细胞的每分钟计数除以悬浮嗜酸性粒细胞的计数来获得。组胺显著增加了嗜酸性粒细胞与上皮细胞的黏附，而TNFα或IFNγ并未显著改变黏附情况。吲哚美辛在未处理和组胺诱导的条件下均显著抑制嗜酸性粒细胞与上皮细胞的黏附。地塞米松也降低了黏附，尽管未达到统计学显著性。这些发现表明组胺诱导嗜酸性粒细胞黏附于气道上皮细胞，提示内源性前列腺素在黏附中的作用。
