An improved estimation method for amino alkyl ligand immobilized on matrix beads.

A direct spectrophotometric suspension method for the estimation of immobilized ligands on a gel matrix was developed. The method uses 0.05% agarose solution to prepare a practically stable suspension of various agarose beads. The quantity of agarose beads in the suspension was estimated turbidometrically from the attenuance at 650 nm. The determination of immobilized ligands on agarose beads was performed colorimetrically at the wavelength of the absorption maximum against a control suspension of a similar amount of underivatized agarose beads. This method is more sensitive and simpler than previously known methods. The method is applicable to the estimation of absorption spectra and the measurement of the time course of reactions on agarose beads.