Iconomou L, Kanellaki M, Voliotis S, Agelopoulos K, Koutinas A A
Department of Chemistry, University of Patras, Greece.
Appl Biochem Biotechnol. 1996 Sep;60(3):303-13. doi: 10.1007/BF02783589.
The continuous making of wine by a delignified cellulosic (DC) material-supported biocatalyst is reported. It was prepared by immobilizing the alcohol resistant strain AXAZ-1 on DC material. The product was found suitable for the continuous process in industrial applications. The operational stability was maintained for 2 mo with monitoring the ethanol concentration, wine, and alcohol productivities as well as the stability of oBe density at the outlet. Wine productivity was three- to sixfold higher than obtained by natural fermentation, alcohol concentrations of the wine was in the range of 9.3-11.2% v/v and low volatile acidities of 0.15-0.36 g acetic acid/L were obtained. The effect of total acidity and flow rate of must were also examined. To demonstrate that the operational stability of the bioreactor is due to DC material that promotes the fermentation, and it takes place at even higher ethanol levels, an analogous system of kissiris supported biocatalyst was studied. Likewise, the tolerance in the alcohol concentration, as compared with free cells, were studied by their stability of the activity in the repeated batch fermentation of must.
报道了使用脱木质素纤维素（DC）材料负载的生物催化剂连续酿造葡萄酒的过程。它是通过将耐酒精菌株AXAZ-1固定在DC材料上制备的。发现该产品适用于工业应用中的连续过程。通过监测乙醇浓度、葡萄酒和酒精生产率以及出口处酒精度密度的稳定性，操作稳定性维持了2个月。葡萄酒生产率比自然发酵高三到六倍，葡萄酒的酒精浓度在9.3-11.2%（v/v）范围内，挥发性酸度低至0.15-0.36 g乙酸/L。还研究了葡萄汁总酸度和流速的影响。为了证明生物反应器的操作稳定性归因于促进发酵的DC材料，并且该过程在更高的乙醇水平下也能发生，研究了类似的基斯里丝负载生物催化剂系统。同样，通过在葡萄汁重复分批发酵中活性的稳定性，研究了与游离细胞相比对酒精浓度的耐受性。
