De Menezes Y, De Faria F P, Sesso A
Department of Pathology, Faculty of Medicine, University of São Paulo, Brazil.
J Submicrosc Cytol Pathol. 1996 Oct;28(4):573-82.
Morphometric analysis by both light and electron microscopy was performed in cells from five cases of human, hepatocellular carcinoma (HCC) and in three control cases. In each case, three fragments were examined individually and the following morphometric parameters evaluated: a) nuclear, cytoplasmic and cell volumes; b) volume density and absolute volume of the rough ER, smooth ER, mitochondria, Golgi apparatus, peroxisomes, dense bodies and cytoplasmic matrix; c) surface density, surface/ volume ratio, and total surface area of rough ER, smooth ER and outer mitochondrial membranes. The parameters obtained from HCC cases showed ample scatter of data, all control values lying within the interval between the extreme values for the various parameters. Both the original and the logarithmically transformed data on volume and total membrane surface area of organelles (y) and of the cytoplasmic volume (x) were regressed using first degree regression equations. The original values for volume and total surface area of rough ER, total ER and mitochondria were linearly related to the corresponding values for cytoplasmic volume. The allometric analysis carried out with the logarithms also revealed significant regressions between cytoplasmic volume and smooth ER parameters not detectable when using the original x and y values. It showed, in addition, that in progressively larger cytoplasmic volumes, the cisternae of both rough and smooth ER tend to appear more compacted and a higher portion of the total ER membrane tends to be constituted of smooth ER. Within the wide range of variation in cytoplasmic volume of the HCC cells, the volume and total surface of the organelles do not vary randomly. These data indicate that in the small, normal-sized and large tumoral cells the mechanisms responsible for the cytoplasmic volume and for the corresponding total volume and membrane surface area of each major organelle are interdependent. Such an interdependence gives no support to ideas implying that the variation in size of cancer cells, an element of pleomorphism, would result of anarchical intracellular synthetic and/or degradative conditions.
对5例人类肝细胞癌（HCC）患者及3例对照患者的细胞进行了光镜和电镜形态计量分析。对每个病例的三个组织块分别进行检查，并评估以下形态计量参数：a）细胞核、细胞质和细胞体积；b）粗面内质网、滑面内质网、线粒体、高尔基体、过氧化物酶体、致密体和细胞质基质的体积密度和绝对体积；c）粗面内质网、滑面内质网和线粒体外膜的表面密度、表面/体积比和总表面积。肝癌病例获得的参数显示数据分散性很大，所有对照值都在各参数极值之间的区间内。使用一阶回归方程对细胞器（y）和细胞质体积（x）的体积及总膜表面积的原始数据和对数转换数据进行回归分析。粗面内质网、总内质网和线粒体的体积及总表面积的原始值与细胞质体积的相应值呈线性相关。用对数进行的异速生长分析还显示，细胞质体积与滑面内质网参数之间存在显著回归关系，而使用原始的x和y值时未检测到这种关系。此外，分析表明，随着细胞质体积逐渐增大，粗面和滑面内质网的潴泡往往显得更紧密，总内质网膜的更高比例倾向于由滑面内质网构成。在肝癌细胞细胞质体积的广泛变化范围内，细胞器的体积和总表面积并非随机变化。这些数据表明，在小的、正常大小的和大的肿瘤细胞中，负责细胞质体积以及每个主要细胞器相应总体积和膜表面积的机制是相互依存的。这种相互依存关系不支持那种认为癌细胞大小变化（多形性的一个要素）是由细胞内无秩序的合成和/或降解条件导致的观点。
