Murozono S, Murayama K, Akahane K
J Biochem. 1977 Jul;82(1):53-8. doi: 10.1093/oxfordjournals.jbchem.a131691.
The main fraction, GF-3, separated from SCM-proteins of fowl feather calamus by gel filtration, was separated into seven peaks, C-1 to C-7, by ion exchange chromatography on a DEAE-cellulose column. Each fraction was found to contain two components by polyacrylamide gel electrophoresis. In order to separate these two components, recycling gel filtration on a Sephadex G-75 column was carried out. Two peaks separated from each of four fractions, C-3 to C-6, were termed C-3a and -3b, C-4a and -4b, and so on. Electrophoretic results showed that all the fractions were single protein components. Components a and b of a particular fraction had almost identical electric charge and slightly different molecular size. All components in Group a (C-3a to C-6a) had similar amino acid compositions except for SCM-cysteine content, and similar results were obtained for Group b (C-3b to C-7b). Groups a and b had significantly different amino acid compositions.
通过凝胶过滤从鸡毛羽管的SCM蛋白中分离出的主要部分GF-3，在DEAE-纤维素柱上进行离子交换色谱分离，得到7个峰，即C-1至C-7。通过聚丙烯酰胺凝胶电泳发现每个部分都含有两种成分。为了分离这两种成分，在Sephadex G-75柱上进行了循环凝胶过滤。从四个部分（C-3至C-6）中的每一个分离出的两个峰分别称为C-3a和-3b、C-4a和-4b等。电泳结果表明所有部分都是单一蛋白质成分。特定部分的a和b成分具有几乎相同的电荷和略有不同的分子大小。除SCM-半胱氨酸含量外，a组（C-3a至C-6a）中的所有成分具有相似的氨基酸组成，b组（C-3b至C-7b）也得到了类似的结果。a组和b组的氨基酸组成有显著差异。
