Kinikli G, Tülek N, Sentürk T, Turgay M, Tutkak H, Duman M, Tokgöz G
Department of Immunology, Ankara University School of Medicine, Turkey.
Allergol Immunopathol (Madr). 1996 Mar-Apr;24(2):65-9.
In the first part of this study, peripheral lymphocyte subpopulations and their proliferative response to PHA and allergens were investigated in the 30 patients with allergic rhinitis compared to 20 healthy non-atopic individuals. Data obtained employing a PHA-induced lymphoproliferative response assay revealed that the allergic rhinitis generated significantly less activity than did the normal control group. Significantly decreased ration of CD4+/CD8+ T cells was noted in the patients with allergic rhinitis. Mean values of stimulation indices by allergen extracts were higher in the patients sensitive to same antigen than others especially in concentration of 1000 SQU/ml. Stimulation of active lymphocytes revealed no statistically significant group differences between allergens. In the second part of the study, the early effect of immunotherapy on T cell subsets and lymphocyte proliferative response to PHA and allergens were examined in the peripheral blood lymphocytes of patients. A significant increase in PHA-induced and in allergen induced lymphoproliferative response were observed in all patients after sixth months of immunotherapy. It is concluded that there may be an association between allergic rhinitis and deficiency of circulating CD4+ cells but further studies are required to substantiate this hypothesis.
在本研究的第一部分，对30例变应性鼻炎患者和20名健康非特应性个体的外周淋巴细胞亚群及其对PHA和变应原的增殖反应进行了研究。采用PHA诱导的淋巴细胞增殖反应试验获得的数据显示，变应性鼻炎患者产生的活性明显低于正常对照组。变应性鼻炎患者的CD4+/CD8+ T细胞比值显著降低。对相同抗原敏感的患者，变应原提取物刺激指数的平均值高于其他患者，尤其是在1000 SQU/ml浓度时。活性淋巴细胞的刺激显示，变应原之间无统计学意义的组间差异。在研究的第二部分，检测了免疫疗法对患者外周血淋巴细胞中T细胞亚群以及淋巴细胞对PHA和变应原增殖反应的早期影响。免疫疗法6个月后，所有患者PHA诱导的和变应原诱导的淋巴细胞增殖反应均显著增加。得出的结论是，变应性鼻炎与循环CD4+细胞缺乏之间可能存在关联，但需要进一步研究来证实这一假设。
