Thunnissen F B, Ellis I O, Jütting U
Department of Pathology, University Hospital Maastricht, The Netherlands.
Anal Cell Pathol. 1996 Oct;12(1):13-24.
Interlaboratory quality assurance studies have been conducted for DNA flow cytometry, but not for DNA image analysis systems. The purpose of this study was to investigate if concordance of DNA image analysis systems existed with respect to classification and staining of standardized material. In three separate rounds, human liver cells were measured randomly by means of the image cytometry system present in each participating laboratory. The features integrated optical density (IOD) and AREA were reported. The relationship between the coefficient of variation (CV) of the 2c and 4c peak were compared with three models. In the three rounds the number of participating laboratories was 11, 14 and 11, respectively. Sequential plotting of normalized IOD values yielded useful information about intra-measurement variation. Comparison of measurements in specimens stained in the participating and central laboratory revealed similar CV values. In general, the precision of the instruments, expressed as the 4c/2c and 8c/2c ratios was good. The accuracy of the different laboratories expressed as the CV of IOD for the three rounds varied from 2-17%. The relation of the CVs of the 2c and the 4c peaks was best fit with the model of the addition of two normal distributions. We conclude that interlaboratory comparison of DNA measurements performed on different instruments is certainly feasible and could facilitate improvement in quality standards.
已经针对DNA流式细胞术开展了实验室间质量保证研究，但尚未针对DNA图像分析系统开展此类研究。本研究的目的是调查DNA图像分析系统在标准化材料的分类和染色方面是否存在一致性。在三个不同阶段，每个参与实验室使用各自的图像细胞分析系统对人肝细胞进行随机测量。报告了积分光密度（IOD）和面积等特征。将2c和4c峰的变异系数（CV）之间的关系与三种模型进行了比较。在这三个阶段中，参与实验室的数量分别为11个、14个和11个。对标准化IOD值进行顺序绘图可得出有关测量内变异的有用信息。对参与实验室和中心实验室染色的标本进行测量比较，发现CV值相似。总体而言，以4c/2c和8c/2c比率表示的仪器精度良好。不同实验室的准确度（以三轮测量中IOD的CV表示）在2%至17%之间变化。2c和4c峰的CV之间的关系最符合两个正态分布相加的模型。我们得出结论，在不同仪器上进行的DNA测量的实验室间比较肯定是可行的，并且有助于提高质量标准。
