Amalfitano G, Bessard J, Vincent F, Eysseric H, Bessard G
Laboratoire de Pharmacologie et Toxicologie, Centre Hospitalier Universitaire, Grenoble, France.
J Anal Toxicol. 1996 Nov-Dec;20(7):547-54. doi: 10.1093/jat/20.7.547.
A gas chromatographic technique with flame ionization detection, which is based on a solid-phase extraction (SPE) procedure using mixed-mode SPE columns, for the simultaneous quantitation of dextropropoxyphene and norpropoxyphene in urine is presented. Urine is treated with sodium hydroxide in order to rearrange, by base catalysis, norpropoxyphene to norpropoxyphene amide, which is then extracted with these columns and chromatographed. The method is specific, linear over the range 0-2000 ng/mL, sensitive, and reproducible. The extracts are cleaner than those obtained with traditional liquid-liquid extraction procedure, which is an important feature in view of further mass spectrometric confirmation of narcotics and other drugs.
介绍了一种采用火焰离子化检测的气相色谱技术，该技术基于使用混合模式固相萃取（SPE）柱的固相萃取程序，用于同时定量尿液中的右丙氧芬和去甲丙氧芬。尿液用氢氧化钠处理，以便通过碱催化将去甲丙氧芬重排为去甲丙氧芬酰胺，然后用这些柱进行萃取并进行色谱分析。该方法具有特异性，在0 - 2000 ng/mL范围内呈线性，灵敏且可重现。提取物比用传统液 - 液萃取程序获得的提取物更纯净，鉴于对麻醉药品和其他药物进行进一步的质谱确证，这是一个重要特征。
