Chiarotti M, Strano-Rossi S, Offidani C, Fiori A
Institute of Legal Medicine, Catholic University of Sacred Heart, Rome, Italy.
J Anal Toxicol. 1996 Nov-Dec;20(7):555-8. doi: 10.1093/jat/20.7.555.
Toxicological analysis of hair was used to detect cocaine use in a group of 615 pregnant women. Hair samples were washed, enzymatically digested, and analyzed by radioimmunoassay for cocaine. Positive results were confirmed by gas chromatography-mass spectrometry after a solid-phase extraction. Benzoylecgonine (BZE) and cocaine were detected after derivatization with pentafluoropropionic-anhydride-pentafluoropropanol. Deuterated cocaine and BZE were used as internal standards. This study demonstrated a mean frequency of 1.9% cases positive for cocaine in all the hair samples examined. The positive rate was 6% among women admitted for spontaneous abortion (66 cases), and the positive rate was 1.4% for those who carried to full term (549 cases). These data underline the usefulness of hair analysis for the diagnosis of drug abuse and demonstrate that there is significant use of cocaine in the Italian population.
对615名孕妇进行头发毒理学分析以检测可卡因使用情况。头发样本经清洗、酶消化后，采用放射免疫分析法检测可卡因。固相萃取后，通过气相色谱 - 质谱联用仪确认阳性结果。用五氟丙酸酐 - 五氟丙醇衍生化后检测苯甲酰爱康宁（BZE）和可卡因。氘代可卡因和BZE用作内标。本研究表明，在所检测的所有头发样本中，可卡因阳性病例的平均频率为1.9%。自然流产孕妇（66例）中的阳性率为6%，足月分娩孕妇（549例）中的阳性率为1.4%。这些数据强调了头发分析在药物滥用诊断中的有用性，并表明意大利人群中存在大量可卡因使用情况。
