Kumar A, Toscani A, Rane S, Reddy E P
The Fels Institute for Cancer Research and Molecular Biology, Temple University School of Medicine, Philadelphia, PA 19140, USA.
Oncogene. 1996 Nov 7;13(9):2009-14.
In this study, we have isolated the genomic DNA clone encoding the murine JAK3 gene and determined its sequence. Partial genomic clones of the JAK1 and JAK2 genes encoding the tyrosine kinase domain were also isolated and compared with JAK3. The genomic structure of JAK3 consists of 23 exons. The exon/intron boundaries and the distribution of coding sequences within the exons of each of the three JAK genes were found to follow a similar pattern suggesting that various members of the JAK family have originated from a single primordial gene by duplication and the structure has been closely maintained through evolution. Using in situ hybridization and FISH analysis, we have mapped the JAK3 gene to human chromosome 19p13.1-p13.2.
在本研究中，我们分离出了编码小鼠JAK3基因的基因组DNA克隆并测定了其序列。还分离出了编码酪氨酸激酶结构域的JAK1和JAK2基因的部分基因组克隆，并与JAK3进行了比较。JAK3的基因组结构由23个外显子组成。发现这三个JAK基因各自外显子内的外显子/内含子边界和编码序列分布遵循相似模式，这表明JAK家族的各个成员通过复制起源于单一原始基因，并且该结构在进化过程中得到了紧密维持。通过原位杂交和荧光原位杂交（FISH）分析，我们已将JAK3基因定位于人类染色体19p13.1 - p13.2。
