Pötsch L, Moeller M R
Institute of Forensic Medicine, Mainz, Germany.
J Forensic Sci. 1996 Jan;41(1):121-5.
This paper represents an experimental approach of histology of the human hair fiber in dyeing and diffusion phenomena and its contribution to the interpretation of hair analysis results for drug abuse. Rhodamine B was applied to human hair fibers from either aqueous solution or methanol/ethanol solvent. The experiments were performed on natural hair of different ethnic groups as well as on extensively bleached hair strands. The microscopical study of the pathway of diffusion of rhodamine B into the hair fibers indicated that the reagent had entered the unmodified fibers at the scale edges between the cuticle cells. At the beginning of the diffusion process intercellular diffusion was the preferred route predominantly along the nonkeratinous regions of the cell membrane complex (CMC) and intermacrofibrillar cement. Penetration into the high sulfur regions of the fiber occurred as dyeing in aqueous solution proceeded and resulted in evenly stained cross sections. The dye distribution pattern observed in natural hair exposed to nonaqueous solution showed that rhodamine B did not penetrate the cortex cells as easy as from aqueous solution and selectively stained nonkeratinous regions only. The determination of the amount of dye taken up by the fibers by spectrophotometric analysis demonstrated that samples diffusion generally increased by time and temperature. It also depended on the morphology of the hair sample. The penetration of rhodamine B from aqueous solution was much greater than from methanol/ethanol solvent.
本文介绍了一种关于人发纤维在染色和扩散现象方面的组织学实验方法，及其对解释药物滥用毛发分析结果的贡献。罗丹明B通过水溶液或甲醇/乙醇溶剂应用于人发纤维。实验在不同种族的天然头发以及经过充分漂白的发丝上进行。对罗丹明B扩散进入毛发纤维途径的显微镜研究表明，该试剂在角质形成细胞之间的鳞片边缘处进入未改性的纤维。在扩散过程开始时，细胞间扩散是主要沿着细胞膜复合体（CMC）的非角质区域和大纤维间黏合质的首选途径。随着水溶液染色的进行，染料渗透到纤维的高硫区域，导致横截面均匀染色。在暴露于非水溶液的天然头发中观察到的染料分布模式表明，罗丹明B不像从水溶液中那样容易穿透皮质细胞，而仅选择性地染色非角质区域。通过分光光度分析确定纤维吸收的染料量表明，样品扩散通常随时间和温度增加。它还取决于毛发样品的形态。罗丹明B从水溶液中的渗透比从甲醇/乙醇溶剂中的渗透大得多。
