Predecki P, Russell P A
J Biomed Mater Res. 1977 Sep;11(5):767-86. doi: 10.1002/jbm.820110511.
The feasibility of preventing platelet adhesion to porous foreign surfaces in contact with blood by passing (perfusing) a physiologic fluid through the pores into the blood was tested. Porous-walled tubes of Teflon (Gore-tex) and aluminosilicate ceramic were perfused with lactated Ringer's solution at rates between 3.13 and 0.3 cc/min per cm2 of perfused surfaced and exposed to heparinized and unheparinized flowing human whole blood for periods of 3-6 min. Under these conditions, with 0.159 cm I.D. tubes and a mean blood flow rate of 8.3 cm/sec, the adhesion of all blood cells was prevented, compared with the adhesion of 1 to 8 X 10(6) platelets/cm2 on the nonperfused controls. The adhesion of plasma proteins was also markedly reduced as determined by bromophenol blue staining. The critical perfusion rate to prevent platelet adhesion to Gore-tex (0.5 micron pore size, 60% pore volume) was found to be between 0.04 and 0.3 cc/(min-cm2). The boundary layer produced by a perfused segment of porous tubing prevented platelet adhesion for several centimeters downstream from the perfusing segment under experimental conditions used.
通过将生理流体经孔隙灌入血液，来防止血小板黏附到与血液接触的多孔异物表面，这种方法的可行性得到了测试。用乳酸林格氏液以每平方厘米灌注表面3.13至0.3立方厘米/分钟的速率，灌注聚四氟乙烯（戈尔特斯）和硅铝酸盐陶瓷制成的多孔壁管，并将其暴露于肝素化和未肝素化的流动人体全血中3至6分钟。在这些条件下，对于内径为0.159厘米的管子，平均血流速度为8.3厘米/秒，所有血细胞的黏附均被防止，相比之下，未灌注的对照样本上每平方厘米有1至8×10⁶个血小板黏附。通过溴酚蓝染色测定，血浆蛋白的黏附也显著减少。发现防止血小板黏附到戈尔特斯（孔径0.5微米，孔隙率60%）的临界灌注速率在0.04至0.3立方厘米/（分钟·平方厘米）之间。在所使用的实验条件下，多孔管灌注段产生的边界层在灌注段下游几厘米处防止了血小板黏附。
