Bailey D S, Northcote D H
J Cell Sci. 1977 Feb;23:141-9. doi: 10.1242/jcs.23.1.141.
A wall/plasma membrane unit was prepared from Hydrodictyon africanum by microdissection. A replica of the inner surface of the membrane was made and by freeze-fracture of the whole cell, 2 corresponding internal fracture faces were obtained. The large coenocytes of the alga were plasmolysed and the wall separated by cutting it away. Its inner surface was directly viewed in the electron microscope after shadowing with Pt/C. Particles were found on the outer half of the internal fracture face of the membrane which were oriented in the same 2 directions as the microfibrils laid down at the inner surface of the wall. No structures were found at the inner surface of the membrane. Some evidence was obtained for a structural connexion between the innermost layers of the wall and the plasma membrane.
通过显微解剖从非洲水网藻制备了壁/质膜单元。制作了膜内表面的复制品，并通过对整个细胞进行冷冻断裂，获得了两个相应的内部断裂面。将藻类的大型多核细胞进行质壁分离，并通过切割将壁分离。用铂/碳进行阴影处理后，在电子显微镜下直接观察其内表面。在膜的内部断裂面的外半部分发现了颗粒，这些颗粒的取向与壁内表面上的微纤丝的两个方向相同。在膜的内表面未发现结构。获得了一些证据表明壁的最内层与质膜之间存在结构连接。
