酿酒酵母FLO1基因座突变体的分离与鉴定。

Isolation and characterization of a mutant of Saccharomyces cerevisiae affected in the FLO1 locus.

作者信息

Reboredo N M, Sieiro C, Blanco P, Villa T G

机构信息

Departamento de Microbiología, Universidad de Santiago de Compostela, Spain.

出版信息

FEMS Microbiol Lett. 1996 Mar 15;137(1):57-61. doi: 10.1111/j.1574-6968.1996.tb08082.x.

DOI:10.1111/j.1574-6968.1996.tb08082.x

PMID:8935657

Abstract

A non-flocculent strain of Saccharomyces cerevisiae was selected after EMS mutation of a flocculent and heterozygous FLO1 locus diploid. The analysis of 25 asci from this diploid showed in all cases segregation 0F:4NF, thus confirming that it was probably affected in the desired gene. After sporulation and dissection of asci, three haploid strains were chosen, which were altered in the locus FLO1. Crossing these three strains with two other ones having markers for ADE1 and pho11::LEU2, we could map the mutation at ca. 4.3 cM and ca. 37.7 cM from the PHO11 and ADE1 loci respectively.

摘要

对一株絮凝型且FLO1位点杂合的二倍体酿酒酵母进行EMS诱变后，筛选出了一株非絮凝型菌株。对该二倍体的25个四分体进行分析，结果显示在所有情况下均出现0F:4NF的分离比，从而证实其可能在目标基因上受到了影响。在四分体形成孢子并进行解剖后，选择了三个在FLO1位点发生改变的单倍体菌株。将这三个菌株与另外两个带有ADE1和pho11::LEU2标记的菌株进行杂交，我们可以将该突变分别定位在距PHO11和ADE1位点约4.3 cM和约37.7 cM处。