Martínez-Campa C, Herrero P, Ramírez M, Moreno F
Departamento de Bioquímica y Biología Molecular, Universidad de Oviedo, Spain.
FEMS Microbiol Lett. 1996 Mar 15;137(1):69-74. doi: 10.1111/j.1574-6968.1996.tb08084.x.
lacZ fusions of the hexokinase 2 gene promoter were constructed and a deletion analysis was performed in order to identify the cis-acting regulatory elements of the promoter that controls hexokinase 2 gene expression. Expression of the hexokinase 2 gene is induced by glucose and around 40-fold repressed by ethanol. This repression seems to be mediated mainly by a repression element located within the coding region of the hexokinase 2 gene, between +39 and +404 bp from the ATG start codon. A second repressing element for ethanol growing cells was located between -455 bp and -254 bp. A synergistic effect on repression of transcription, when ethanol is the carbon source used for growth, was demonstrated by experiments in which both repressing elements were simultaneously removed. The finding of regulatory sequences in the coding region of the hexokinase 2 gene stimulates a search for regulatory elements in the coding region of other yeast genes.
构建了己糖激酶2基因启动子的lacZ融合体，并进行了缺失分析，以鉴定控制己糖激酶2基因表达的启动子顺式作用调控元件。己糖激酶2基因的表达受葡萄糖诱导，而受乙醇抑制约40倍。这种抑制似乎主要由位于己糖激酶2基因编码区内、距ATG起始密码子+39至+404 bp之间的一个抑制元件介导。乙醇生长细胞的第二个抑制元件位于-455 bp和-254 bp之间。通过同时去除两个抑制元件的实验证明，当乙醇作为生长所用碳源时，对转录抑制具有协同作用。在己糖激酶2基因编码区发现调控序列，促使人们在其他酵母基因的编码区寻找调控元件。
