Biagini R E, Driscoll R J, Bernstein D I, Wilcox T G, Henningsen G M, MacKenzie B A, Burr G A, Scinto J D, Baumgardner E S
Department of Health and Human Services, Public Health Service, National Institute for Occupational Safety and Health, Cincinnati, OH 45226, USA.
J Appl Toxicol. 1996 Mar-Apr;16(2):139-45. doi: 10.1002/(SICI)1099-1263(199603)16:2<139::AID-JAT321>3.0.CO;2-P.
Thirty-six employees who produced industrial enzymes from selected strains of bacteria and fungi were evaluated by epicutaneous threshold testing and enzyme-linked immunosorbent assays (ELISA) for specific IgE and IgG antibodies. The workers complained of 'asthma- and flu-like' symptoms, which generally lessened away from work. The enzymes evaluated were: alpha-amylase (1,4-alpha-d-glucan glucanohydrolase) from Bacillus licheniformis (alpha ABl), B. subtilis formation 1 (alpha A1Bs) and B. subtilis formation 2 (alpha A2Bs); purified alpha-amylase from B. licheniformis (C alpha ABl) and A. oryzae (C alpha AAo); alkaline protease from B. licheniformis (APBl) and purified alkaline protease (CAPBl); amyloglucosidase (1,4-alpha-d-glucan glucohydrolase) from A. niger (AGAn) and purified amyloglucosidase (CAGAn). Statistically significant increases (P > 0.05) in the proportion of workers having positive skin tests to CAPBl, AGAn and CAGAn were found. Significantly elevated (P > 0.05) mean specific IgE results were observed for C alpha AAo CAGAn and AGAn, and elevated (P > 0.05) mean specific IgGs were observed for C alpha AAo, CAGAn, AGAn, alpha A1Bs, alpha AB1 and alpha A2Bs. These results indicate that occupational exposure to some industrial enzymes can cause immediate-onset cutaneous hypersensitivity reactions, pulmonary function deficits and significantly elevated specific antibody levels. Our results are equivocal as to whether work-related respiratory and cutaneous hypersensitivity reactions are antibody mediated, as there was no statistically significant association between these reactions and specific IgE or IgG levels.
通过表皮阈值测试和酶联免疫吸附测定（ELISA）对36名使用选定细菌和真菌菌株生产工业酶的员工进行了特定IgE和IgG抗体评估。这些工人抱怨有“类似哮喘和流感”的症状，这些症状通常在离开工作场所后会减轻。所评估的酶包括：地衣芽孢杆菌的α-淀粉酶（1,4-α-D-葡聚糖葡聚糖水解酶）（αABl）、枯草芽孢杆菌形态1（αA1Bs）和枯草芽孢杆菌形态2（αA2Bs）；地衣芽孢杆菌（CαABl）和米曲霉（CαAAo）的纯化α-淀粉酶；地衣芽孢杆菌的碱性蛋白酶（APBl）和纯化碱性蛋白酶（CAPBl）；黑曲霉的淀粉葡糖苷酶（1,4-α-D-葡聚糖葡糖水解酶）（AGAn）和纯化淀粉葡糖苷酶（CAGAn）。发现对CAPBl、AGAn和CAGAn皮肤试验呈阳性的工人比例有统计学显著增加（P>0.05）。观察到CαAAo、CAGAn和AGAn的平均特异性IgE结果显著升高（P>0.05），CαAAo、CAGAn、AGAn、αA1Bs、αAB1和αA2Bs的平均特异性IgG升高（P>0.05）。这些结果表明，职业性接触某些工业酶可导致速发型皮肤过敏反应、肺功能缺陷以及特异性抗体水平显著升高。关于与工作相关的呼吸道和皮肤过敏反应是否由抗体介导，我们的结果并不明确，因为这些反应与特异性IgE或IgG水平之间没有统计学显著关联。
