Suzuki K, Okumura Y, Sato T, Yasuda T, Oki A, Oki M, Sunamoto J
Department of Synthetic Chemistry and Biological Chemistry, Graduate School of Engineering, Kyoto University, Japan.
Transfusion. 1996 Nov-Dec;36(11-12):966-8. doi: 10.1046/j.1537-2995.1996.36111297091739.x.
Some of the major blood group antigens are on lipids and proteins of the red cell membrane. Incubation of intact red cells with liposomes containing specially designed artificial lipids has been shown to result in the extraction of membrane proteins by the liposomes. The extraction of blood group structures and the retention of their antigenicity have not been reported.
After the incubation of red cells with liposomes, the extraction of the antigens from human red cells by liposomes was examined by evaluation of the agglutination of the liposomes by respective antisera.
Agglutination specific to the A and B blood group antigens was seen, which indicated that the antigenicity of the blood group antigens was retained even after the extraction by the liposomes. The presence of an artificial boundary lipid, 1,2-dimyristamido-1,2-deoxyphosphatidylcholine, in the liposome was crucial to the efficient extraction of the A and B antigens. On the other hand, the extraction of D, M, N, and P1 was not always detectable by agglutination.
The A and B blood group antigens were directly extracted from red cells by liposomes without loss of antigenicity.
一些主要血型抗原存在于红细胞膜的脂质和蛋白质上。已证明完整红细胞与含有特殊设计人工脂质的脂质体孵育会导致脂质体提取膜蛋白。尚未有关于血型结构提取及其抗原性保留的报道。
红细胞与脂质体孵育后，通过评估各抗血清对脂质体的凝集作用，检测脂质体对人红细胞抗原的提取情况。
观察到针对A和B血型抗原的特异性凝集，这表明即使在脂质体提取后，血型抗原的抗原性仍得以保留。脂质体中人工边界脂质1,2 - 二肉豆蔻酰胺 - 1,2 - 脱氧磷脂酰胆碱的存在对于高效提取A和B抗原至关重要。另一方面，通过凝集作用并非总能检测到D、M、N和P1的提取。
脂质体可直接从红细胞中提取A和B血型抗原，且不丧失抗原性。
