Sex steroids regulate the expression of plasminogen activator inhibitor-1 and its mRNA in fibroblasts derived from uterine endometrium.

In order to clarify a role of stromal cells in sex steroidal neovascularization, plasminogen activator inhibitor (PAI)-1 [an inhibitor of tissue-type plasminogen activator (tPA) and urokinase-type plasminogen activator (uPA)] and its messenger ribonucleic acid (mRNA) were analysed in fibroblasts derived from uterine endometrium as a model for endometrial stromal cells under the influence of sex steroids. The determinations were carried out by an enzyme-linked immunosorbent assay (ELISA) and reverse transcription-polymerase chain reaction-Southern blotting, respectively. In the fibroblasts, either estradiol or progestogens (progesterone, medroxy progesterone acetate or 17 alpha-hydroxyprogesterone) induced expressions of PAI-1 and its mRNA, and their combination further increased their expression by approximately twofold. PAI-1 from endometrial stromal cells under the influence of sex steroids might contribute to endometrial neovascularization through its effect on endothelial cells in endometrial vessels.